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文献和实验The ribonuclease protection assay (RPA)
, 5X transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32
Native Chromatin Preparation and Illumina/Solexa Library Construction
(1X PBS containing 0.5 % bovine serum albumin, filtered) T4 DNA ligase (400 U/µL) and 10X buffer Taq polymerase (New England Biolabs) TaqMan PCR master mix (Applied Biosystems) (see Step 38) TE (1X, pH 7.4) Triton X-100
Construction and Characterization of Adenovirus Vectors
cells (~90% confluence) with 106 pfu in 250 µL of MEM. Infect a second 60-mm dish with 107 pfu in 250 µL of MEM. Infect a 150-mm dish with 108 pfu in 1 mL of MEM. Return cells to the incubator. xiv. Allow the virus to adsorb
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