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文献和实验Transformation of E. coli by Electroporation
of the Chamber safe and secure it with the draw latch. 5. Plug the pulse cable into the right side of the Chamber safe. 6. Turn the chamber selection knob on top of the Chamber Safe to direct the electrical pulse to the desired Micro-Electroporation
Procedures Common to the First Five Behavioral Tests
to avoid any sharp click from the door latch. Transport to and from apparatus When all is ready, remove the cage top and place it on a towel to avoid sharp noise. [The details of the next step will vary for the different tests. ]
before experiement) take out filtered gel aliquot and fluorescent dye from fridge next door 30min ahead of time (1 gel aliquot tube enough for 2 chips) take out ladder from fridge upstairs vortex dye 10s and spin down mix one tube gel
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