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文献和实验Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM
. Transfer 1.5 ml to a micro centrifuge tube and spin 2 min. Decant the supernatant. Repeat with another 1.5 ml of cells. Drain well onto a Kimwipe. 2. Resuspend the pellet in 467 μl TE buffer by repeated pipetting. Add 30 μl of 10% SDS and 3 μl
are precipitated with sodium acetate, and the lysate is cleared first by filtration of precipitate through cheesecloth and then by centrifugation. The DNA-containing supernatant is transferred to a new tube, and the plasmid or cosmid DNA is precipitated
cDNA Synthesis from MOLT-4 Cells
cDNA sample to the center øf the top frit and let it drain into the bed. Collect the effluent into tube 1. 4) Add 100 μl of Ten buffer to the column, and collect the effluent intotube 2.(Note: Let the column drain completely before the addition of new
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