- ￥858
- Beckman Coulter贝克曼库尔特
- 美国
- A98814
- 2025年07月15日
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文献和实验or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
Construction and Characterization of Adenovirus Vectors
before use. Equipment Agarose gel electrophoresis equipment Bottles (polypropylene, 500- and 1000-mL) Centrifuge (high-performance) (e.g., Avanti J-series, Beckman) Cryovials (4-mL) Dialysis cassettes (Slide-A-Lyzer, 10,000-kDa
Preparation of BAC DNA with by Alkaline Prep Method
within few days. *****Other prep sizes may change yields and purity***** 2. Transfer the culture to a 250 ml centrifuge bottle (polypropylene, washed and autoclaved; lids scrubbed with Alconox) and spin at 6000 g's for 15 minutes at 4℃(fixed angle; e.g. JA-14
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