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- Beckman Coulter贝克曼库尔特
- 美国
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- 2025年07月16日
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文献和实验the plasmid DNA of interest into a 17 X 100 mm Falcon tube containing 6 ml of TB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 16-18 hours with shaking at 250 rpm. 2. Harvest the cells
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
EGTA Store at 4 deg C; (we generally make and store as a 5X stock) Cytoskeleton Buffer (CB) with sucrose (CBS) 10mM MES pH 6.1 138mM KCl 3mM MgCl 2mM EGTA Store at 4 deg C Add sucrose fresh on the day of use of the buffer
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
80mM PIPES pH 6.8 1mM MgCl2 1mM EGTA Store at 4 deg C; (we generally make and store as a 5X stock) Cytoskeleton Buffer (CB) with sucrose (CBS) 10mM MES pH 6.1 138mM KCl 3mM MgCl
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