- ￥9152
- Beckman Coulter贝克曼库尔特
- 美国
- C35991
- 2025年07月08日
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文献和实验to hydrolyze DNA, using an RNAse free DNAseI, and purify the cleaned RNA free of DNA and free of DNAseI activity.ProcedureTransfer the total RNA to a clean 1.5 ml tube and adjust the volume of the total RNA sample to 100 ul with RNAse free water.Add 350 ul
2. Grind 2 g of fresh, leaf tissue to a powder in Liquid Nitrogen in a chilled mortar and pestle. 3. Scrape the powder into a chilled 50 ml tube. 4. Add 3 to 5 ml CTAB Buffer per gram tissue to the tube. 5. Incubate the sample at 60℃
We suggest the following procedure for total RNA isolation based on years of experience working with complicated samples and small amounts of starting material (Matz, 2002). The procedure is suitable for all types of tissues from wide
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