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- Beckman Coulter贝克曼库尔特
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- B85899
- 2025年07月08日
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文献和实验Mag-Bind Blood RNA Isolation Protocol (for 50ul Blood)
, skip this DNase I digestion steps (step 14-21) and proceed step 17 for isolating both DNA and RNA. 11. Add 50 μl of DNase I digestion mix and resuspend the magnetic particles by pipetting up and down for 20 times or vortexing. Incubate at room
Single tube confirmation PCR protocol
(200mg/l). - Incubate at 30°C for two to three days. *This step reduces background by eliminating aborted transformants. 2. Zymolyase treatment - Transfer a small portion of a well-isolated colony (~1 mm) into 50 µl solution
High Throughput Isolation Of PCR Products Using ChargeSwitch® PCR Clean-Up
. For more information, see http://www.invitrogen.com or call Technical Service. Follow the protocol below to purify PCR products. The volumes given are on a per sample basis. 1. To ~50 µl PCR samples in 96-well plates, add 10 µl ChargeSwitch® Magnetic Beads.
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