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文献和实验Introduction Extraction and purification of plasmid DNA from E.coli can be performed on a small scale,where DNA is extracted from just 1-2 ml of bacterial culture (mini-prep), or from much larger volumes (100 ml %26ETH; maxi-prep; 1 L %26ETH
RNA Isolation From Animal tissue or cell culture
实验试剂 Regents supplied by user: 1. 96-100% ethanol 2. $-Mercaptoethanol 实验设备 Equipments supplied by user: 1. RNase-free filter pipette tips 2. Microcentrifuge capable of 12,000
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
the medium, and re-suspend the cells in an appropriate amount of fibroblast medium. d. Count the cells using the desired method (e.g., Countess® Automated Cell Counter), and seed the MEF culture dishes with 5 × 104 –2 × 105 cells per 100-mm dish
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