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- Beckman Coulter贝克曼库尔特
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- 2025年07月15日
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文献和实验. 5. Cap the tubes and centrifuge briefly to collect the contents 6. Incubate tubes in a thermal cycler at 94°C for 30 s to 2 min to completely denature the template and activate the enzyme. 7. Perform 25-35 cycles
Extraction of DNA using DNAzol® Reagent
. Use a loosely fitting homogenizer, with a tolerance of 0.1-0.15 mm or higher. Homogenize 25-50 mg tissue in 1 ml of DNAzol® Reagent by applying as few strokes as possible. Typically, 5-10 strokes are required for complete homogenization. Small amounts
ES CELL DNA EXTRACTION: TUBE ME
in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this way! Lysis buffer: ( 100 ml Recipe ) 10 mM Tris-HCl pH7.5 - 0.5 ml of 2M 10 mM EDTA - 2 ml of 0.5M 10 mM NaCl - 0.2 ml
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