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- Beckman Coulter贝克曼库尔特
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- 2025年07月12日
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文献和实验Myosin Light Chain Preparation
. Day 6 and after 23.Centrifuge in J21℃using JA20 rotor at 15,000 rpm for 20 minutes.Take a small sample for gel and assays and freeze the remainder in -60℃ freezer (note volume). 24.Determine the protein concentrations of Pools
. If desired, the tubes may be left on ice overnight. 9.Pellet the phage-PEG complex by centrifugation at 10,000 rpm for 15min at 4℃. Use of a swinging bucket rotor (HB-4) is convenient for this step because the pellet will be concentrated
Protocols for ET recombination
synthesis, the EMBL oligo service prefers that the 5' most nucleotide is a T or C. Consequently most of our oligos start with a T or C however we do not think this is important for ET cloning efficiencies). The 3' end (the PCR primer) - choose about 18-30
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