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文献和实验Tracking individual chromosomes with integrated arrays of Lacop sites and GFP-Laci repressor
Introduction The visualisation of specific DNA sequences in living cells, achieved through the integration of lac operator arrays (lacop ) and expression of a GFP-lac repressor fusion, has provided new tools to examine how the nucleus
,通常需要20000美元左右哦。采用单波长LED模块设计,输出单个波长的荧光谱线,例如365nm/488nm/561nm/633nm, 类似于激光光源。此类优点突出;寿命长,采用光电快速切换,有效避免物理震动。LED单模块光源的单色性好,极大的降低了白光光源的背景噪音,高效激发。程序控制适合整合到高端的成像系统中。例如:EXCELITAS公司X-CITE TURBO系统,蔡司公司的Colibri LED光源即属于此类代表,寿命普遍在几千个小时。适用染料LED模块波长DAPI385CFP430GFP
ASTRACT We describe a rapid and quantitative flow cytometric method for determining the apoptotic or anti-apoptotic potential of a gene in various cell types. A plasmid carrying green fluorescent protein (GFP) is co-transfected
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