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文献和实验under the base of a sterile plate. Typically, the grids contain 24 or 50 squres, but grid between 9 and 121 squares have been used. Use a grid system of the appropriate size for the number of strains to be printed. 4. Pick a single colony of each strain
Hints and precautions for the care, feeding and breeding of Neurospora
and quickly. Most auxotrophs and some morphological mutants can be distinguished from their wild counterparts, and counted or isolated selectively. Ascospores are taken by loop from a cross tube to a drop of water in the middle of the 3 agar plate; the desired
3' overhang makes a small contribution to the specificity of target recognition restricted to the unpaired nucleotide adjacent to the first base pair. 2'-Deoxynucleotides in the 3' overhangs are as efficient as ribonucleotides, but are often cheaper
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