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文献和实验.7. Incubate the plate right side up at 37°C. Plaques should appear in 4 hours, and lysis should approach confluence in 5 to 7 hours.8. When lysis is just confluent, pipette 2 ml of lambda-diluent onto the plate. Scrape up the top agar overlay, and chop
.Decant, add 250 mls fresh NaCl/NaOH, rotate 20 minutes.5. Decant NaCl/NaOH. Wash briefly with ddH2O.6. Neutralization: Place gel in 250 mls of 3M NaCl 0.5M Tris, pH 7.0. 500 mls = 88 g NaCl 3g Tris base 36 g Tris HCl Rotate gently 20 minutes. Decant
base + 36 g Tris HCl Rotate gently 20 minutes. Decant, add 250 mls fresh NaCl/Tris, rotate 20 minutes. 7. Measure gel exactly. Cut off bottom right corner. Put gel back in neutralization solution. Cut nitrocellulose paper about 1 mm shorter than gel
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