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文献和实验Procedure Dilute p27 monoclonal antibody 1:1000 (v/v) in Carbonate Coating Buffer. Add 100 µl/well and incubate o/n @ 4°C or 1 hr @37°C. Wash 3X with TBST (pour onto plate, empty into sink, hit onto towel 3x to clear wells
An Ultra-fast method of DNA extraction from Neurospora
the need for large numbers of flasks when growing many cultures for DNA isolation. 1. Inoculate plastic Petri plates containing 25 ml of 1X Vogels, 2 sucrose, 0.04 Tween-80 (to prevent conidiation) (Zalokar, M. 1954. Arch. Biochem. Biophys. 50:71-80
ES CELL DNA EXTRACTION: TUBE ME
in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this way! Lysis buffer: ( 100 ml Recipe ) 10 mM Tris-HCl pH7.5 - 0.5 ml of 2M 10 mM EDTA - 2 ml of 0.5M 10 mM NaCl - 0.2 ml
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