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- Beckman Coulter贝克曼库尔特
- 美国
- C14295
- 2025年07月08日
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文献和实验101 culture in 50 ml of 2xTY, as above. 2. Using sterile toothpicks, transfer individual M13 plaques into 12 X 75 mm Falcon tubes containing 1 ml early log phase cell cultures, and incubate for 4-6 hours at 37degC with shaking at 250 rpm. (Growth
media. 6 3. Insert the tip of a sterile pipette into the stock bottle and remove 10 ml of media. Transfer the media to the tissue culture flask. 4. Open the top of the suspension culture and use a sterile 1.0 ml transfer pipette to remove a 1.0 ml
Hints and precautions for the care, feeding and breeding of Neurospora
it tightly to the glass. We decided to use plain 13 X 100 mm culture tubes with cotton plugs and parafilm seals for silica gel stocks, in preference to screwcap tubes. With the latter, we could not be confident that the seal was tight, and the constricted
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