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文献和实验media. 6 3. Insert the tip of a sterile pipette into the stock bottle and remove 10 ml of media. Transfer the media to the tissue culture flask. 4. Open the top of the suspension culture and use a sterile 1.0 ml transfer pipette to remove a 1.0 ml
101 culture in 50 ml of 2xTY, as above. 2. Using sterile toothpicks, transfer individual M13 plaques into 12 X 75 mm Falcon tubes containing 1 ml early log phase cell cultures, and incubate for 4-6 hours at 37degC with shaking at 250 rpm. (Growth
101 culture in 50 ml of 2xTY, as above. 2. Using sterile toothpicks, transfer individual M13 plaques into 12 X 75 mm Falcon tubes containing 1 ml early log phase cell cultures, and incubate for 4-6 hours at 37degC with shaking at 250 rpm. (Growth
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