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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
PS-1 1 1" diameter post fork Newport PS-F 1 Achromatic Zero-Order
The MTT Cell Proliferation Assay
with the procedure and know the cell count to use in your specific assay, you may follow this basic protocol.1 、Plate cells at 1000 to 100,000 per well.2 、Incubate for 6 to 24 hours3 、Add 10 μl MTT Reagent4 、Incubate for 2 to 4 hours until purple precipitate
Lacrimal Experimental Protocol (4/4/94)
and make up in 8 ml of DMEM/gent. plus goodies. Take 15 µl of cell suspension plus 15 µl of trypan blue, mix gently then add 15 µl to each side of the hemocytometer. Count # of dead (blue) and living cells in grid using 10 x objective lens. Cell number
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