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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
1 High-Energy Nd:YAG Laser Mirror, 25.4 mm Diameter, 45°, 532 nm Newport 10Q20HE.2 4 SUPREMA Optical Mount, 1.0 inch diameter
光滤色镜为例,介绍一下滤色镜组的构造以及根据染料,如何正确选择滤色片。 荧光激发块是荧光显微镜中用来激发荧光标本和观察荧光的核心部件,它实际是滤色片的组合,一般含有3种滤色片: 1. 激发滤色片(Exciter Filter):选择透过某个波段的光,来激发荧光染料。 2. 分色镜 (Dichroic Mirror):反射激发光,透过荧光,一般激发光波长短,而发射光波长较长。 3. 发射滤色片(Barrier Filter) :透过发射光,也就是我们看到的“荧
Using Cell‐ID 1.4 with R for Microscope‐Based Cytometry
Image splitter with an appropriate dichroic mirror and top and bottom emission filters, e.g., for CFP/YFP FRET, a Dual View image splitter (Optical Insights/Photometrics, http://www.photometrics.de) fitted with the following filters
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