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文献和实验Use of the B.D. FACS or Calibur Flow Cytometers
then you should use the tool palette or the "Plots" menu to create FSC vs SSC Dot Plots and Histogram plots of FL1, FL2, or FL3 as necessary. If multicolor flow is being performed then Dot plots of FL1 vs FL2, etc. should also be set up to aid in adjusting
of analysis. 2. Quantification by flow cytometry Analyze Annexin V-FITC binding by flow cytometry (Ex = 488 nm; Em = 530 nm) using FITC signal detector (usually FL1) and PI staining by the phycoerythrin emission signal detector (usually FL
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
to the dish, which will limit the number of OP9 cells that are transferred. 35. Wash the non-adherent cells off the dish, filter the cells through a 40-µm cell strainer, and centrifuge the cells at 400g (1500 rpm) for 5 min at 4°C
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