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文献和实验Transgenic Outline: Mice and Rats
of vector sequences in not required for BAC transgenes. Transgene constructs are then quantitated and microinjected into (C57BL/6 X SJL)F2 mouse eggs and surgically transferred to recipients. This is a fee for service provided by the Transgenic Core
Preparation of NeoR Murine Embryonic Fibroblasts
anesthesia) or CO2 asphyxiation. Prepare three 10cm dishes containing 15ml PBS each. With the mouse on its back, wipe down the abdominal skin and fur with 70% alcohol. Make an incision down the midsection using scissors, thereby exposing the uterine horns
Preparation of NeoR Murine Embryonic Fibroblasts
, 15ml/dish. Place the decapitated embryos in the dish containing Trypsin/EDTA. Using curved Iris scissors finely mince the tissue quickly until it can be taken up in a 10ml pipette. Pipette up and down 6-7 times and place the dish of minced embryos
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