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文献和实验in 24-well-plate at concentration of 100,000 cells / well, each sample in triplicate. One day before assay: centrifuge cells, bring back to volume with Fischer's SM (stationary medium). It is important to remove all of MM from cells
75), assay will be performed in 24-well-plate at concentration of 100,000 cells / well, each sample in triplicate. One day before assay: centrifuge cells, bring back to volume with Fischer's SM (stationary medium). It is important to remove all of MM
ES CELL DNA EXTRACTION: TUBE ME
Protocol for extracting DNA from ES Cells, starting from the 96-well plate but processing in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this way! Lysis buffer: ( 100 ml Recipe ) 10 mM
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