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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
at room temperature or overnight at 4°C with gentle shaking. Drain off excess solution. 27. Add 40 µL of diluted affinity-purified primary antibody to each slide, and cover with a coverslip. Incubate the slides for 1 h at room
In Situ Hybridization Using Digoxigenin Labeled Probes
the chromosomes for this may obscure the diaminobenzidine precipitate 11) Place a drop of mounting media over the chromosomes and cover with a 22 mm square coverslip 12) Examine the chromosomes using phase-contrast optics. Hybridization signals appear
to be living are the bacteria. The smallest bacteria can be observed and cell shape recognized at a mere 100x magnification. They are invisible in bright field microscopes, though. These pages will describe types of optics that are used to obtain contrast
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