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文献和实验Isolation of colonic epithelium
by grasping the cecum with forceps and pulling until it is straight. Then cut close to the cecum and the rectum. 2. Flush the colon using a 10 ml syringe and a large needle, preferably with the point cut off, eg. #16. This is to remove the contents
Isolation of colonic epithelium
by grasping the cecum with forceps and pulling until it is straight. Then cut close to the cecum and the rectum. 2. Flush the colon using a 10 ml syringe and a large needle, preferably with the point cut off, eg. #16. This is to remove the contents
Preparation of a DNA Sequencing Gel
Acrylamide Mix (50% Acrylamide) 3.0 mL of 10x TBE buffer ddH2 O to 25 mL Mix until the urea is completely dissolved. 2) Filter the gel mix using a 0.22 um pore filter unit and a 30 mL syringe. 3) Degas the gel mix. - Transfer
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