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文献和实验Fluorochrome Absorption and Emission Spectra
, which are similar in structure to phycoerythrobilin, the chromophore in R-PE. It has a 650-nm wavelength absorption maximum (Fig. 2) and a 660-nm fluorescence emission maximum (Fig. 2). Using a 660 ?10 nm BP filter will give optimum detection for this fluorochrome
Fluorochrome Absorption and Emission
, which are similar in structure to phycoerythrobilin, the chromophore in R-PE. It has a 650-nm wavelength absorption maximum (Fig. 2) and a 660-nm fluorescence emission maximum (Fig. 2). Using a 660 ?10 nm BP filter will give optimum detection for this fluorochrome
). Staurosporine (800 ng/ml) was added following transfection as indicated (+ staurosporine). Excitation wavelength of 488 nm was used for eGFP and PI, 647 nm for Cy5 and multiline UV for DAPI. Emmision fluorescence was collected using a 530/20 nm bandpass filter
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