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文献和实验Overview This is a method to assemble two BioBricks using the Clontech In-Fusion PCR Cloning Kit and maintains BioBrick standard formats. There are currently several BioBrick assembly standardsthat all involve assembly by restriction enzyme
Chromatin Assembly Using Drosophila Systems
chromatin structure and activity in vitro, it is essential to have a chromatin assembly system that will prepare extended nucleosome arrays with highly defined protein content that resemble bulk chromatin isolated from living cell nuclei in terms
CSF Extract Prep for Spindle Assembly
that we find are most important for obtaining good CSF extracts that are competent for CSF spindle assembly and for cycled spindle assembly. The indicated buffer amounts are sufficient for a 4 frog prep. For sperm nucleus preparation, see above protocol. For spindle assembly
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