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文献和实验Fluorochrome Absorption and Emission Spectra
daltons and an absorption maximum at 495 nm (Fig. 2). Its excitation by 488-nm light leads to a fluorescence emission maximum around 520 nm (Fig. 2). Using a 530 ?15 nm bandpass (BP) filter will give optimum detection for this fluorochrome
Fluorochrome Absorption and Emission
daltons and an absorption maximum at 495 nm (Fig. 2). Its excitation by 488-nm light leads to a fluorescence emission maximum around 520 nm (Fig. 2). Using a 530 ?15 nm bandpass (BP) filter will give optimum detection for this fluorochrome
, resuspend in 2 ml 1 x LiAc/ 0.5 x TE incubate at RT for 10 min combine in tube: 100 µl cells, 10 µl salmon sperm DNA (10 mg/ml), up to 15 µl DNA to be transformed add 700 µl 1 x LiAc / 1 x TE / 40 % PEG mix incubate for 30 min
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