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- Beckman Coulter贝克曼库尔特
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- A11895
- 2025年07月16日
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
Cellular lysis solution ChIP diluent Cloning vector The vector used should have enzyme overhangs similar to those generated in the 3C assay. Such vectors can be custom-constructed or obtained commercially. For example, for cloning
Introduction: C. elegans RNA preps that have been widely used previously involved fairly slow lysis of the worms, potentially leading to degradation of the RNA. In addition, they failed to separate RNA from genomic DNA, leading
C. elegans whole mount immunohistochemistry with Bouin's Fixative
). The tube is rocked an additional 30 minutes. A solution of BTB [ 1X Borate buffer, 0.5% Triton X-100, 2% ßME] is made from 50X borate buffer ( 1.0 M H3BO3, 0.5 M NaOH). The worms are spun from fixative in a centrifuge [ 2-3 seconds in an eppendorf 5415C
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