ELBOW, MALE 1/8 PIPE BR.(Servi

Gene Targeting Outline

contribution. ES cells produce agouti fur and the C57BL/6 host cells produce black fur. Another sign that the ES cells have successfully colonized the host embryo is a distorted male:female sex ratio in the chimeras. Since ES cells are X:Y, a good clone

Mouse Breeding

(many female chimeras and few male chimeras). You probably injected a X:O cell line. The females may very will give germline transmission. 2. Breed as many males as you can, we have observed germline transmission from males with small quantities of agouti

SSR GEL and Silver Staining Protocol

Service Research 5985 Highway K Rhinelander, WI 54501 USA Phone: 715-362-1121 Fax: 715-362-1166 e-mail: pmarquar@newnorth.net I. EQUIPMENT: DNA sequencing unit (35 x 45 cm) & 2000V power supply Clamps Lg. plastic trays (4), about 43 x 50 x