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文献和实验CaPO4 Transfection for Chromaffin Ce
with 1.0 M NaOH. Adjust volume to 100 ml. If desired, filter sterilize. Store at 4'C N.B.: The above amounts assume the free acid ot PIPES. For NaPIPES use 1.30g. 4. DNA in TE of H2O 115 ug/ml (this is the optimum amount). Procedure:
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
. References: 1. Hammarlund, M., Nix, P., Hauth, L., Jorgensen, E.M., & Bastiani, M. Axon regeneration requires a conserved MAP kinase pathway. Science. 323, 802-806 (2009). 2.
The ribonuclease protection assay (RPA)
, then spin in a microfuge for 2 minutes at RT. 5. Transfer the upper aqueous phase to a new 1.5 ml Eppendorf tube and add 50 µl 4 M ammonium acetate and 250 µl ice cold 100% ethanol. Invert the tube to mix and incubate for 30 minutes at -70°C. Spin
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