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文献和实验Recommended Reagent Concentrations: Primers: 0.2 - 1.0 uM Nucleotides: 50 - 200 uM EACH dNTP Dimethyl sulphoxide (DMSO): 0 - 10% (v/v) Taq polymerase: 0.5 - 1.0 Units/50ul rxn Target DNA: 1 ng - 1 ug (NB: higher concn for total genomic DNA
Standard Protocols for the Construction of Fab Libraries
Fab libraries, in which light-chain (LC) and heavy-chain (HC) variableregion genes are cloned into a phagemid vector and subsequently displayed on the surface of the filamentous phage particle, have been widely used for the isolation
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
phenol/chloroform/isoamyl alcohol (25:24:1), vortex, centrifuge for 5 min and remove the supernatant to new tubes 11. Repeat step 10 12. Repeat this step with chloroform alone 13. Add 300 l isopropanol and incubate on ice for 10 min 14
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