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- 2025年07月10日
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文献和实验E-Z 96 X-press Plasmid Vacuum Protocol
selective antibiotic. Incubate 14-16 hours at 37℃ 600 with vigorous shaking until an OD of 2.0-4.0 is achieved. 2. Pellet 1-2 mL bacterial culture in a 2.0 ml 96-well deep-well plate by centrifugation at 3000 x g for 15 min at room temperature.
Single tube confirmation PCR protocol
Polymerase. - Add 35 µl of the PCR mix to each of the 20 PCR tubes that already contain the 15 µl of primers and template. 110 µl 5 µl 10 x Taq buffer(see below) 11 µl 0.5 µl 20 mM dNTP's (0.2 mM) 11 µl 0.5 µ
96-well RNA In Situ Hybridization Protocol
Noordermeer and Casey KopczynskiI.PREPARATION OF EMBRYOS1. Embryos were collected for 24 hours and washed 3X with 0.02% Triton X (Tx)(15ml for up to 2ml embryos in a 50ml falcon tube).2. Dechorionate 5 min. in 50% bleach.3. Wash 3X with 0.02% Tx.4. Fill tube
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