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文献和实验Fibronectin Sigma F0895-2MG Glass Bottle (250ml) with Cap Cole Parmer Instrument Co. 34594
, the sonicated human placental DNA can be added during the pre-hybridization to the bag/bottle at a level of 100-200 ug/ml of hybridization solution. Solutions: Church Buffer -- 1 Liter BSA 10 gm 0.5M EDTA 2 ml 1 M NaHPO4(pH: 7.2) 500 ml 20% SDS 350 ML
100 ml, sterile filter to remove particulate matter pour into a bottle, and carefully mark the level of the meniscus Add DEPC to 0.1%, shake to dissolve DEPC Let stand overnight at 37o Autoclave 20 minutes After autoclaving
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