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文献和实验Functional Genomic Analysis in Pain Research Using Hybridization Arrays
is that one interrogates a panel of probes (gene-specific cDNA fragments or gene-specific oligonucleotides that have been immobilized on a solid support) with RNAs (targets) from control and treated experimental samples that have been either radioactively or fluorescently
Using CellX to Quantify Intracellular Events
Figure 14.22.1 Overview of the calibration steps. Blue boxes indicate protocol steps, the red decision box corresponds to visual inspection of the control images by the user, the green circles cover possible troubleshooting steps and adjustment
duplex PCR amplification, which is the coamplification of a fragment from the region of interest and a control fragment (e.g., the actin gene, or the tubulin gene). This approach allows for estimating relative levels of specific histone modifications
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