- ￥11903
- Beckman Coulter贝克曼库尔特
- 美国
- A04-1-0033
- 2025年07月09日
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文献和实验A SENSITIVE METHOD FOR DETECTION OF APOPTOSIS BY SINGLE LASER FLOW CYTOMETRY
immunofluorescence by single laser flow cytometry. J Immunol Meth 170: 145-157, 1994. Staining for detection of apoptosis 1 x 106 PBSAz-washed cells from a single cell suspension are pelleted in a 12 X 75mm culture tube. The pellet is resuspended
制备蛋白提取物1. Prepare extracts from cultured cells or tissues with our Trident Extraction Kits.The total number of cells per ml and the cell equivalent loaded per lane of gel should be optimized specifically for each protein and antibody.2. Determine
Vybrant® DyeCycle Green and Orange Stains
or Vybrant® DyeCycle™ Orange stain from the freezer and allow it to equilibrate to room temperature. Prepare flow cytometry tubes each containing 1 mL of cell suspension in complete media at a concentration of 1 × 106 cells/mL. To each tube
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