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文献和实验Preparation and purification of biotin-labeled probe for ISH
, however the size of the probe is relatively easier to control by nick translation. II. Purification of the biotin-labeled probe. Start 20 min before the end of the nick translation incubation. Plug the bottom of a 1 ml tuberculin syringe
(1000X) 0.5 ml + Glutamine (100X) 5 ml + 5% FBS (25 ml) ProcedureDraw venous blood into 60 ml syringe with 10ml 6% dextran + 7 ml citrate/citric acid. Fill to 60 ml total (blood 43 ml).Mix and sediment 30 min at RT (white blood cells now with serum
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of No. 30 G 1/2 needle is cut off and then rounded using sharpening stone); 1 ml syringe; Dissecting instruments (fine-pointed scissors, fine forceps); No. 5 forceps (Dumont); Mouth pipette (aspirator mouth piece, latex tubing, blue
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