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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
thoroughly with warm water and Alconox (Fisher Scientific); cool by filling with liquid N2 . Remove frozen, flat mycelia from foil and add it to the liquid N2 in mortar. Add ~0.5 g of baked sand and grind mycelial pad to a fine powder. Add more N2 as needed
秀丽线虫RNA提取--C. elegans RNA prep
to use freshly prepared worms, since these can be lysed most quickly, but a flash frozen pellet that was stored at -80° is fine. Do not process more than one 5 ml aliquot of worm suspension at a time; this will entirely fill the SW28.1 rotor for the later
A quick RNA mini-prep for Neurospora mycelial cultures
); cool by filling with liquid N2 . Remove frozen, flat mycelia from foil and add it to the liquid N2 in mortar. Add ~0.5 g of baked sand and grind mycelial pad to a fine powder. Add more N2 as needed. Mortar and pestle should be washed after every sample
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