LID, ROTOR TY-50.2TI(Service)

离心技术基础

CENTRIFUGAL FORCE Figure F.2. Nomogram for R.C.F. Modern day ultracentrifuges can generate forces in excess of 300,000 times that of gravity, forces sufficient to overcome the very cohesion of most molecules (including the metal of the rotor). The force

Centrifugation

and other parameters will vary with the particular instrument and rotor used. Relative Centrifugal Force is a constant that is independent of the apparatus used. Figure F.2 presents a Nomogram for calculation of R.C.F. for a given radius and RPM. A simple formula

Appendix F: Centrifugation

is calculated by:   t = 402/100or 4.02 hours (4 hrs, 1 min) Table F.2 presents the k values for the Sorvall SS-34 rotor and for the Sorvall GSA rotor. Since these two rotors will be used throughout the remainder of the course, reference