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- Beckman Coulter贝克曼库尔特
- 美国
- 356915
- 2025年07月09日
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文献和实验Single tube confirmation PCR protocol
tests are performed on each colony to confirm both of the novel recombination junctions . 1. Clonal purification - Pick three colonies from a transformation plate and streak each one out to single colonies on separate G418 containing plates
by pipetting them up and down three times. Transfer 200 ul of the mixture to the 48-well plate (Plate A). 6. Incubate at 37 degreeC, 5% CO2 for 3 to 30 h. 7. Day 2 and 3: Observe endothelial tube size and numbers under microscope and photograph
ES CELL DNA EXTRACTION: TUBE ME
in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this way! Lysis buffer: ( 100 ml Recipe ) 10 mM Tris-HCl pH7.5 - 0.5 ml of 2M 10 mM EDTA - 2 ml of 0.5M 10 mM NaCl - 0.2 ml
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