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文献和实验A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform
DNA cloning is a basic methodology employed for multiple applications in all life-science disciplines. In order to facilitate DNA cloning we developed Transfer-PCR (TPCR), a novel approach that integrates in a single tube, PCR amplification
Single tube confirmation PCR protocol
(200mg/l). - Incubate at 30°C for two to three days. *This step reduces background by eliminating aborted transformants. 2. Zymolyase treatment - Transfer a small portion of a well-isolated colony (~1 mm) into 50 µl solution
by pipetting them up and down three times. Transfer 200 ul of the mixture to the 48-well plate (Plate A). 6. Incubate at 37 degreeC, 5% CO2 for 3 to 30 h. 7. Day 2 and 3: Observe endothelial tube size and numbers under microscope and photograph
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