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- Beckman Coulter贝克曼库尔特
- 美国
- 001-6588
- 2025年07月07日
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文献和实验Embryo Dissection and Micromanipulation Tools
. All tools must be kept clean and rinsed with 70% ethanol to keep them sterile. RELATED INFORMATION For excellent advice on dissection and micromanipulation tools, see Hamburger (1960) . Forceps Stainless-steel forceps
:1) and gently shake for 2 hrs. Centrifuge at 2,800 rpm for 30 min. Collect supernatant using a sterile transfer pipet (IWAKI 7801-002, cut off 3 cm from the tip). Add 2 ml 10% CTAB solution and mix gently. Add 20 ml 24:1 chloroform : isoamylalcohol and gently
Preparation of Mitochondria from Rat Liver
, and 1 mM EDTA, pH 7.2 for homogenizing. We use a shearing type homogenizer called 'Tissuemizer,' with T25 stainless steel shaft, made by Tekmar, Inc. (Cincinnatti, Oh). A teflon-on-glass (Potter-Elvehjem) homogenizer can also be used, but it takes longer
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