RES, SIP-POT, 10 1/4W, 10%(Ser

Use of a Novel 5′‐Regioselective Phosphitylating Reagent for One‐Pot Synthesis of Nucleoside 5′‐Triphosphates from Unprotected Nucleosides

are building blocks for enzymatic synthesis of DNA and RNA. This unit presents a protocol for convenient synthesis of 2??deoxyribo? and ribonucleoside 5??triphosphates (dNTPs and NTPs) from any natural or modified base. This one?pot synthesis

Two‐Step, One‐Pot Synthesis of Inosine, Guanosine, and 2′‐Deoxyguanosine O6‐Ethers via Intermediate O6‐(Benzotriazol‐1‐yl) Derivatives

) and Cs2 CO3 under a nitrogen atmosphere. Conversion to the O 6 ?(benzotriazol?1?yl) ethers occurs within about 10 min for inosine, and within about 60 min for guanosine and 2??deoxyguanosine. Then, for reaction with alcohols, the reaction mixture

Arabidopsis transformation by floral dipping（拟南芥转化）

or add 10% bleach). Use a 25 ml pipette to transfer about 20 ml of the inoculation medium to each centrifuge bottle. Resuspend the cells, first by mixing them with the tip of the pipette, then by pipetting up and down. When they are resuspended