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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
for 1.5-mL tubes (Invitrogen) Microcentrifuge, refrigerated Microscope, inverted Pasteur pipettes Pipettors and tips, 5- to 1000-µL Refrigerator or cold room preset to 4°C Rotating wheel/platform Shaking incubator preset
.[Autoradiography: A technique where radioactively labelled molecules are visualised through exposure to X-ray film.The PCR steps are all carried out, one after the other, in bouts of cycling. Cycle 1 is as follows: • During denaturation (about 1 min at 95°C
Kingfisher Flex 96 Plant DNA High Pure Protocol
实验试剂 Regents to be provided by user 1. Absolute (96%-100%) ethanol 实验设备 Equipments to be provided by user 1. Centrifuge capable of at least 3,000-5,000 x g 2. Rotor adapter for 96
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