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文献和实验two to three colonies from each agar plate and set upa small volume (typically 5 ml) LB culture for miniprep. Incubate and shake under 37 °C overnight, prepare plasmidDNA using a spin miniprep kit according to manufacturer’sprotocol. 6. (Optional) Digest
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
through a 0.45 mm filter and concentrated by centrifugation in an SW27 rotor at 4°C, 20K, for 2 hours. The concentrated stock was titered on QT6 and tested for helper virus, which proved negative. The titer of CHAPOL was determined to be 1.1 x 107 CFU/ml
FACS Procedures for Apoptosis Detection
, although it is very difficult to obtain tight CVs. The protocol below is carried out in 96-well V-bottomed micro titer plates (MTP); with some modifications, it could be carried out in 12 x 75 mm plastic tubes. Phoenix Flow Systems has a protocol for pre-fixing samples
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