ARM, LOCKING(Service)

ET recombination

of problems is poor quality of the synthesised oligonucleotides. Now that the EMBL oligonucleotide service has been dropped and we are forced to rely on commercial sources, we know that the quality of oligonucleotides is critical and can vary wildly

Protocols for ET recombination

Oligo design The 5' end (the homology arm) - choose 42 or more (we usually choose about 50) nts for the homology arms from the target DNA sequence simply according to where you want to insert the PCR fragment. (For efficient oligonucleotide

High Throughput Isolation Of PCR Products Using ChargeSwitch® PCR Clean-Up

handling robotic workstation with a gripper arm to process samples in 96-well plates. Appropriate tips for liquid dispensing and aspiration [You may use any tips of choice to dispense and aspirate liquid during the purification procedure. Consider