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文献和实验Construction and Characterization of Adenovirus Vectors
2006 ). Suspend the DNA in a total volume of 25 µL of 0.1X TE-Ad. 12. Repeat Steps 5-10 with 5 µL of DNA (from Step 11) and 100 µL of DH5 cells, rather than BJ5183. 13. Select two colonies from each plate. Purify the DNA
Protocols for ET recombination
the cells at 2.3 kV (Bio-Rad Gene Pulser), 25 uF with Pulse controller set to 200 ohms) Add 1 ml of LB medium and transfer back into the eppendorf tube. Incubate at 37℃ for 1 to 1.5 hours with shaking. Plate 100ul on suitable antibiot℃ plates, spin
SSR GEL and Silver Staining Protocol
Published in: Echt CS, May-Marquardt P, Hseih M, Zahorchak R. 1996. Characterization of microsatellite markers in eastern white pine. Genome 39:1102-1108 . comments can be directed to Paula Marquardt at: USDA Forest Service Research
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