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文献和实验Transspecies Grafting as a Tool to Understand the Basis of Murine Developmental Limb Abnormalities
, proliferative state. Surgical extirpation of the AER results in truncated limbs; the most distal structures formed can be correlated with the time of AER removal (5 ). Thus, in the characterization of abnormal phenotypes in which the limb fails to develop
SDS-PAGE Western Blotting Protocols
is the same as the that of the spacers’. 6. Remove comb. 7. Pour resolving gel to the mark in step 5. Optional: Create an agarose plug with newly liquified 1% agarose (i.e., the agarose needs to be hot when used). The agarose does not affect the running of the SDS
Preparation of Double/Single-Stranded DNA and RNA Molecules for Electron Microscopy
. The electron microscope, as a tool for analyzing molecular structure, interactions, and processes, is one that can be used with increasing confidence, particularly in conjunction with biochemical and biophysical studies (1 -4 ). In order to prepare nucleic
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