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文献和实验SDS-PAGE Western Blotting Protocols
the screws. If the assembly is correct,then the whole set-up should snap into place when placed above the gray gasket. 4. Remove alignment card. 5. Slide in comb and mark a line at 2-3cm from the bottom of the comb. Make sure that the comb thickness
Preparing and Running a Protein Gel (7% Polyacrylamide)
plate faces the apparatus and the foam blocks form a seal with the gasket, and clamp it onto the gel apparatus using spring clips on the sides. 4) Pour 1x Running Buffer into the upper buffer chamber of the gel apparatus and check for leaks
Lipoprotein Analysis Week 2: Electrophoresis
of residual acry-lamide to insure a good seal. Place the silicone rubber gaskets on top of the red foam pads of the casting stand slots. 6. Transfer the clamp assembly to one of the casting slots in the casting stand. If two gels are to be cast, place
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