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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
twice with 0.2 mL of 1X PBS to remove free IgGs (5 min each wash). 32. Remove the supernatant from the beads. Add 500 µL of chromatin extract (from 107 cells) to the beads in each tube. Rotate at 4°C overnight. 33. Wash
Preparation of Carboxypeptidase Y and Its Properties
2. Reagents ⑴ DEAE-ion exchange cellulose (DE-32): Weigh out the appropriate amount of DE-32 for the required bed volume of your column. Swell it in distilled water completely, and then decant the small particles that can not precipitate, then suction
while working as a chemist at the Cetus Corporation, a biotechnology firm in Emeryville, California. The procedure requires placing a small amount of the DNA containing the desired gene into a test tube. A large batch of loose nucleotides, which link into exact
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