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文献和实验Whole Mount X-gal Histochemistry of Transgenic Animal Tissues
the post-fixed tissue to 70% ethanol for long term storage (up to several years). The stain is stable and ethanol whitens the tissue for better contrast in whole mount photos. Long term storage (longer than a couple weeks) in formalin can harden the tissue
Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)
, since the effect is obtained by selective reduction of illumination. Exercise: observing pseudopodia of Chaos carolinensis Prepare a vaseline mount of Chaos carolinensis (also called Pelomyxa Carolinensis ). Chaos is a large amoeboid protist
mount is extremely thin, dark field mode may distort details. However the various organelles show wide variation in refractive index, that is, the tendency of the materials to bend light, providing an opportunity to distinguish them. Highly
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