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文献和实验Transformation of E. coli by Electroporation电穿孔转化E.coli
. 12.Turn power switches on for both the power booster and pulse control apparati. Voltage booster will display -16.66. 13.Charge pulse control by setting the CHARGE/ARM switch to CHARGE, then press and hold down the UP voltage control button
chambers (cuvettes) •Growth plates with proper antibiotic for plasmid. •Plating rod •37 ℃ shaking incubator and regular incubator. Procedure 1.Be sure power is off on the voltage booster and pulse control apparati
Use of the B.D. FACS or Calibur Flow Cytometers
reservoir of the cytometer with PBS before getting started. The Beckman Calibur cytometers have a green power button on the right side. There is also a second power toggle switch hidden on the back of the machine. Boot the computer after the cytometer
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