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文献和实验Chick Chorioallantoic Membrane (CAM) Assay
containing major vessels. The embryos were then returned to the humidified 38°C incubator with 3% CO2 for 24 to 48 additional hours. Visualization of vessels Embryos were removed from the incubator and meshes were viewed under a dissecting microscope
Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
慢病毒转染肝细胞方法Lentivirus Transduction of Hematopoietic Cells
. These cell types are refractory to most nonviral or retroviral delivery methods. Lentiviral vectors are capable of transducing nondividing cells and maintaining long-term and sustained expression of the transgenes. Many hematopoietic cell types
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